介绍JotDyes: 纯净精确的膜标记

JotDyes 突出表现为全球首款能够无缝覆盖整个脂质双分子层的膜标记染料，提供了前所未有的细节和准确度水平。

传统染料通常存在挑战，需要在类似二甲基亚砜或乙醇等亲脂性溶剂中重新配制。由于它们缺乏生物相容性，这些溶剂可能会对生物样本引入不必要的改变。此外，一些染料在稀释过程中有形成纳米聚集物的倾向，可能会导致假阳性，特别是在分析诸如细胞外囊泡（EVs）等小生物颗粒时。

然而，JotDyes经过工程设计，仅在嵌入脂质双分子层后才会发出光亮，在避免聚集的同时，由于其水溶性，可以避免聚集。这一独特特征不仅增强了生物相容性，而且简化了工作流程，确保以无与伦比的效率消除假阳性。

JotDyes为广泛的标记应用提供了一个优越选择，涵盖了从EVs到哺乳动物细胞的范围。

细胞外囊泡

这种水溶性的JotDyes能够准确检测纳米级别的细胞外囊泡、外泌体和脂质体，不会产生人为成分或假阳性结果。其专利化学成分可以防止自组装成胶束或纳米颗粒。这些高发光染料在>2mg/mL的水溶缓冲液中溶解，在靶向结合后才会发出荧光。已验证适用于20多种细胞系的EV类型，例如MSC、PC3、HT29、A549，以及红细胞等体液。

水溶性（> 2 mg/mL 可溶性） 稳定标记（> 24 小时） 无细胞间染料交换 短孵育时间（约 30-60 分钟） 提供多种颜色可选

代表性数据:

Flow cytometry analysis of EVs labelled with JotDye. Dot plot of (a) unstained EVs, (b) EVs labelled with 1 μM JotDye, (c) 1 μM of JotDye only and (e) 1 μM of commercially available PKH26 dye only.

Imaging flow cytometry. PC3 EVs were labeled with 2 µM JotDye and purified using the Amicon 100KDa filter before they were added to A549 cells and incubated. The cells were analyzed on the Imagestream (Amnis) after (a) 2 h, (b) 4 h and (c) 8 h of incubation. The JotDye-labeled exosomes were observed as spots (green). In the absence of EVs, no fluorescence signal was observed in the cells because all the free JotDye, which does not form micelles, were removed from the purification step (d). By contrast, micelles of DiD dye were not purified and thus there was still fluorescence signals being observed from the cells, suggesting the uptake of the DiD micelles which can be mistaken for DiD-labeled EVs instead.

哺乳动物细胞

细胞分裂监测是免疫调节、细胞功能、静止状态、增殖和分化等细胞生物学研究的重要组成部分。荧光细胞分裂追踪剂通常用于流式细胞术和成像，以研究体内外不同细胞类型的相互作用和命运。Jotbody膜染料可用于跟踪细胞分裂，因为它们与哺乳动物细胞结合稳定且细胞毒性低。这些染料还具有非常高的量子效率，可以实现长时间的荧光跟踪。

代表性数据:

	Membrane labelling Confocal images of HepG2 cells that were co-stained with Jotbody Membrane Labelling Dye and a commercially available membrane dye, FM 4-64. (a) JotDye channel (Ex405), (b) FM 4-64 channel (Ex488), (c) brightfield channel, (d) merged channel. The co-localization of JotDye and FM4-64 demonstrates the membrane specificity of the JotDye.
	Cell division tracking A549 mammalian tumor cells labeled by Jotbody Cell Division Tracking dye. JotDye-labeled cells (red) have a 3-log fold increase in fluorescence intensity compared to the unstained cells (black) were maintained over 4 passages through serial passaging before analysis on CytoFLEX (Beckman Coulter). Orange: 1st passage, Green: 2nd passage, Blue: 3rd passage. Magenta: 4th passage.
	Red blood cell labelling Flow cytometry analysis of samples that have a mixture of unstained red blood cells (RBC) and RBC stained with JotDye, in the ratio of (a) 0:5, (b) 2:3, (c) 3:2 and (d) 5:0. The JotDye for RBC remains stable in the cell after labelling and does not cross-over into samples that are not intended to be labelled with the dye. (e) Bright field and (f) fluorescent confocal micrograph of RBCs stain with JotDye.

为什么选择 JotDyes？

1. 荧光亮度: JotDyes以高量子产率（>25%）著称，只有在与目标结合时荧光才会增强。这种荧光变化是由周围环境的变化触发的，支撑了独特的“点亮”机制。
2. 多样的色彩调色板: 凭借多功能的色团平台，JotDyes提供多达4种不同颜色，持续努力拓展激发/发射谱。这种多样性为各种应用打开了新的多重标记可能性。
3. 水溶性: 与在水溶液中易于形成胶束或纳米颗粒的染料不同，JotDyes保持高度水溶性。这种特性确保了准确的分析，特别是在细胞外囊泡的流式细胞术评估中，假阳性可能会扭曲结果。

精选产品:
JotDye-430 Excitation= 355 nm (UV laser), Emission= 403 – 460 nm
JotDye-490 Excitation = 405 nm (violet laser), Emission = 458 – 508 nm
JotDye-600 Excitation = 488 or 561 nm (blue or yellow laser), Emission = 586 – 635 nm
JotDye-800 Excitation = 638 nm (red laser), Emission = 775-818 nm

拥抱精准。拥抱纯净。拥抱 JotDyes — 用无与伦比的清晰度照亮您的研究。