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Nanobody
Discovery
Discovery
Nanobodies originate from the variable domain of heavy chain antibodies in camelids (camels and llamas) or cartilaginous fish (sharks and rays), with a molecular weight of only 12-15 kDa, making them the smallest known antibody fragments (2-4 nm). They are characterized by their small size, high stability, excellent specificity, high affinity, low immunogenicity, ease of genetic modification, and scalability in production. These advantages confer significant value in basic research, clinical diagnostics, and therapeutics.
Technical Process
Service Advantages
- Diverse Animal Sources: Covering camels, alpacas, llamas, sharks, with rich immune strategies.
- Comprehensive Technology Platform: Encompassing target preparation, antibody screening, antibody expression, antibody optimization platforms.
- Delivery of 20+ Sequences: Ensuring specificity of CDR sequences.
- Independent Intellectual Property Rights: Antibody sequence intellectual property rights belong to the client.
- Efficient Delivery: Delivery completed in as fast as 3 months.
- Strict Quality Control: Rigorous quality control at every step.
Service Offerings and
Duration
| Stage | Service content | Service type | Time frame | Deliverables |
|---|---|---|---|---|
| I | Antigen preparation | Proteins, peptides, cells, DNA, etc. | ~3 weeks | Preparation by us or provided by the client |
| II | Animal immunization | Alpacas, camels, sharks, etc. | ~8 weeks | Serum, PBMC, antibody titer report |
| III | Library construction | Naïve library, immunized library | ~2 weeks | Phage display library construction, library quality assessment report |
| IV | Antibody screening | Solid-phase, liquid-phase, negative screening, competitive screening, cell screening, etc. | ~2 weeks | Enriched positive nanobody sequences after selection |
| V | Antibody evaluation | ELISA/SPR/FC/IF, etc. | ~3 weeks | Nanobody expression and activity validation |
| VI | Report compilation | Data analysis of all above stages | ~1 week | Final project report |
| Duration | Naïve library discovery: 1.5~2 months; Immunized library discovery: 3~5 months (camel-derived), 5~7 months (shark-derived). | |||
Naïve library characteristics: Derived from hundreds of healthy, non-immunized camelid animals (3/5 from alpacas, 1/5 from llamas, 1/5 from camels), library size 4.7×10^11 (evaluated by NGS), VHH insertion rate ~100%, library is available for sale.
Validation Data
